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Next monoclonal antibodies was in fact purchased of Pharmingen (Hillcrest, Calif Phone staining and disperse cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA] and 0.01% NaN3) with optimal amounts of [...]